Mechanistic Studies on Malate Dehydrogenase from Escherichia coli
نویسندگان
چکیده
منابع مشابه
Mechanistic studies on malate dehydrogenase from Escherichia coli.
Kinetic studies and chemical modification studies using diethylpyrocarbonate and iodoacetate were performed on malate dehydrogenase isolated from Escherichia coli. Product inhibition experiments indicate that this enzyme follows an ordered Bi Bi kinetic mechanism, similar to other dehydrogenases, while log V/K profiles reveal that one ionizing group with a pKa between 7.8 and 8.7 acts as a gene...
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The structure of apo malate dehydrogenase from Escherichia coli has been determined to 1.45 A resolution. The crystals belonged to space group C2, with unit-cell parameters a = 146.0, b = 52.0, c = 168.9 A, beta = 102.2 degrees. The structure was determined with the molecular-replacement pipeline program BALBES and was refined to a final R factor of 18.6% (R(free) = 21.4%). The final model has ...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1995
ISSN: 0003-9861
DOI: 10.1006/abbi.1995.1397